| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Thiol- and Biotin-Labeled Probes for Oligonucleotide Quartz Crystal Microbalance Biosensors of Microalga Alexandrium Minutum
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| Autores: |
Lazerges, Mathieu
Perrot, Hubert
Rabehagasoa, Niriniony
Compere, Chantal
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| Data: |
2012-07-04
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| Ano: |
2012
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| Palavras-chave: |
Alexandrium Minutum
Avidin
Biosensor
Biotin
DNA
Quartz crystal miocrobalance
Self-assembled monolayer
Thiol
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| Resumo: |
Two quartz crystal microbalance oligonucleotide biosensors of a toxic microalga gene sequence (Alexandrium Minutum) have been designed. Grafting on a gold surface of 20-base thiol- or biotin-labeled probe, and selective hybridization with the complementary 20-base target, have been monitored in situ with a 27 MHz quartz crystal microbalance under controlled hydrodynamic conditions. The frequency of the set up is stable to within a few hertz, corresponding to the nanogram scale, for three hour experiments. DNA recognition by the two biosensors is efficient and selective. Hybridization kinetic curves indicate that the biosensor designed with the thiol-labeled probe is more sensitive, and that the biosensor designed with the biotin-labeled probe has a shorter time response and a higher hybridization efficiency.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00107/21810/19385.pdf
DOI:10.3390/bios2030245
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| Editor: |
MDPI
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| Relação: |
http://archimer.ifremer.fr/doc/00107/21810/
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| Formato: |
application/pdf
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| Fonte: |
Biosensors (2079-6374) (MDPI), 2012-07-04 , Vol. 2 , N. 3 , P. 245-254
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| Direitos: |
2012 by the authors; licensee MDPI, Basel, Switzerland
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